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Poster Presentations

 

 

POSTER 6

Epifauna on Freshwater Crayfish (Crustacea: Decapoda) in Croatia

Dražina T, Maguire I, Korša A, Špoljar M and Klobučar G

The crustacean exoskeleton is well known substratum for associate species - epibionts. During 2014 and 2015, we have studied epifauna on six different freshwater crayfish species collected from continental and mediterranean regions of Croatia (Europe). Four of them are autochthonous (Astacus astacus, Astacus leptodactylus, Austropotamobius pallipes, Austropotamobius torrentium) and two allochthonous invasive species (Pacifastacus leniusculus and Procambarus fallax f. virginalis). The epibiont samples were collected from the exoskeleton surface and from the crayfish gill cavity. The aims of this research were: (i) to analyse epifaunal assemblage on different freshwater crayfish and (ii) to compare epifauna among different crayfish populations, especially between autochthonous and allochthonous species. A total of 44 different epifaunal taxa were recorded. The most abundant group was Ciliophora, with Vorticella campanula and Epistylis sp. as the most frequent taxa. Rotifera was the most diverse group and 16 different taxa were identified. Four rotifer species (Lepadella astacicola, L. branchiola, L. parasitica and Dicranophorus hauerianus) were determinated as specific inhabitants of certain crayfish species and these rotifers were found exclusively on autochthonous species. Branchiobdellidans were constant crayfish epibionts, and for the first time the North American species Xirogiton victoriensis has been recorded in Croatia. Representatives from Catenulida, Gastrotricha, Nematoda, Bivalvia, Hirudinea, Tardigrada, Crustacea, Hydrachnidia and Chironomidae were also recorded as epibionts on freshwater crayfish. Results of this study suggested the separation of epifaunal assemblage between autochthonous and allochthonous crayfish, as well as the separation of epifauna among autochthonous species. Our results indicate highly diverse, complex and specific epifaunal assemblage on different crayfish populations. Thus, multiple relationships between crayfish host and epibionts will be the topic of further studies.

 

 

POSTER 7

The stone crayfish in the czech republic: lost-and-found in last seven years

Vlach P, Fischer D and Svobodová J

Within the period 2012-2015, an intensive research concerning: 1. mapping the occurrence of stone crayfish; 2. monitoring of presently known populations; 3. an evaluation of ecological demands of this species, was carried out. This contribution focuses on newly described localities, losses of some populations, and populations affected by a dramatic decrease in population densities in some localities. Moreover, the contribution introduces a hypothesis or/and particular reasons for that decrease or extinction. The extinction in Úpor brook and Hýskovský brook has already been published; recently we have recorded the following losses: Zákolanský brook, Bertínský brook, Vlcí brook, Kornatický brook, Hrádecký brook, and Medvedí brook. Whereas the combination of crayfish plague and (probably) low water quality caused the total mortality in lower part of the Zákolanský brook, crayfish plague outbreak killed crayfish in Kornatický and Hrádecký brook in 2015. Conversely, there was a lethal concentration of BOD5 in Vlcí brook. Also dramatic droughts in 2015 influenced the population densities in Chocenický and Prešínský brook. The mass mortality in Klabava was caused by a combination of stream acidification and toxic metal accumulation. The reasons for other losses are mostly speculative. On the other hand, within the mapping campaign, we found more than 10 new populations of stone crayfish. Nevertheless, the newly recorded occurrences correspond with a present range of this species in the Czech Republic.

 

 

POSTER 8

Fine Structure of the Spermatozoon in Three Species of (Arthropoda: Crustacea: Decapoda) Cambarus robustus, Orconectes propinquus and Orconectes rusticus: A Comparative Biometrical Study

Yazicioglu B, Hamr P, Kozák P, Kouba A and Niksirat H

The ultrastructure of spermatozoa in three species of cambarid crayfish, including Cambarus robustus, Orconectes propinquus, and Orconectes rusticus were studied and compared with eight previously studied species from different crayfish families using morphological features and biometrical data. The ultrastructure of spermatozoa show a generally conserved pattern including an acrosome and nucleus in the anterior and posterior parts of the cell, respectively, radial arms that wrap around the nucleus, and the whole cell is enclosed by an extracellular capsule. The most outstanding morphological feature in spermatozoa of three studied cambarid crayfish is the crest-like protrusions in the anterior part of the acrosome that can be used as one of the features for distinguishing the members of this family. Results of biometrical data reveal that acrosome size in the representatives of Parastacidae are the smallest, while representatives of Astacidae show the biggest acrosome. The acrosome size in species belonging to Cambaridae occupy an intermediate position between the two other families of freshwater crayfish. In conclusion, a combination of morphological features and biometrical data of spermatozoa can provide an effective tool to distinguish different species of the freshwater crayfish.

 

 

POSTER 9

Metabolic Rate of Cambarellus montezumae (Crustacea: Cambaridae): Effects of Size and Seasonal Changes

Latournerié-Cervera JR, García-Padilla GM, and Estrada-Ortega AR

The measurement of metabolic rate is a key element of a species energy balance. The evaluation of respiratory metabolism in natural conditions, allows us to know the energy requirement of the animal for different processes including growth, activity, reproduction, among others. The objective of this study was to know the element (R) of the energy budget equation: C = P+R+F+U (IBP, 1968) in Cambarellus montezumae, during hot rainy season (May – August) and transitional months (September – October) in the area of study. Recently collected samples of crayfish (n=15) representing small, medium and large sizes of both sexes were employed in respirometry experiments, using a closed respirometer. The temperature and water quality were similar to the conditions of the sampling site. Two measurement periods were used: (noon and evening). Average routine metabolic rate was used for comparisons (QO₂: mg O₂/g. dry weight/h) and was converted to cal/g.dw/day using the Q_ox=3.31 cal/mgO₂ (Brafield & Solomon, 1972). Comparisons for size and month were made by ANOVA factorial (3 X 6) and Tukey test post hoc (p<0.05). Significant differences were obtained by month and size. QO₂ was similar between the months of the rainy season, with a temperature average range of (22.3 ± 1.6°C) and had a significant decrease (p<0.05) in October. Relationships of QO₂ – WW were calculated for all months using a potential model. We discuss results in relation to energy requirements of the species for growth and reproduction and its potential use for intensive management.

 

 

POSTER 11

Life History and Population Ecology of Signal Crayfish, the New Invader in Northern Italy

Ghia D, Fea G, Gruppuso L, Bo T, Candiotto A, Fenoglio S and Sacchi R

The occurrence of the signal crayfish Pacifastacus leniusculus in the Valla stream, north-western Italy, was one of the first reports of this invasive alien species in Italy. Since 2009 signal crayfish was spreading upstream from the artificial lake formed by a dam for hydroelectric purpose. We studied the signal crayfish population in this novel environment by trapping sessions during a year. Five sampling sites were located along the invaded range of the Valla stream, along more than 10 km. At each site, six baited traps were set once a month for two days, from April 2015 to March 2016. All signal crayfish were removed from the watercourse. Crayfish were sexed, weighted and the following morphometric parameters were measured: total length (TL), and cephalothorax length (CL). We trapped overall 433 females (mean TL: 84.7 mm) and 428 males (mean TL: 85.5 mm). Crayfish activity was correlated with water temperature. Egg-bearing females occurred from early November and hatching in late May. Sex ratio was about 1:1 in all sites except the most downstream one, where females were more abundant; and more females were caught during summer months. Crayfish TL and condition factor fluctuated during the year, but both decreased significantly moving upstream. Management actions should be promoted to limit its spreading overall within watercourses potentially suitable.

 

 

POSTER 12

Analysis of Abundance, Fecundity and Allometric Relationships from Cambarellus montezumae Females During an Annual Cycle

Latournerié-Cervera JR, García-Padilla GM, Estrada-Ortega AR and Arana-Magallón F

Cambarellus montezumae population at Xochimilco, Mexico City, is in serious problems of disappearance. This species was highly appreciated and it was regularly consumed as part of the diet of the Xochimilcas and Mexican people. Nowadays, crayfish is very scarce in this area. As a part of a collaboration research, UNAM and UAM universities have undertaken the project of assessment and rescue of native species in the area of Xochimilco. In this study, we analyze the female population of C. montezumae during an annual cycle. 42 berried females were collected in ten months of sampling (January – October). Abundance varied significantly by sampling month (p<0.05). 76.2% of females were collected during hot rainy season (April – August). Description and analysis of these females were made through: total and cephalothorax length, (TL and CTL). Large and width of abdomen, wet weight, ovigerous mass weight and number of eggs. Minimum, maximum and average values for TL, WW and number of eggs were: (28.31, 43.21, 34.5) mm, (0.58, 1.91, 0.99) g, and 13, 150 and 46.9 eggs respectively. Distributions of TL and WW were positive skewness. Allometric relationships were calculated for WW – TL (potential model, r²=0.93), CTL – TL (linear model, r²=0.86). Fecundity – TL was positive correlated (r=0.68, p<0.05). Also positive and significant correlations (p<0.05), were obtained for abdomen width and length – TL and female abundance – temperature. Ovigerous mass weight was higher on April and number of eggs per female was bigger on September. We discuss results in relation to scarcity of berried females, the decrease in the number of eggs per female, the size of first maturity, and anthropogenic impacts in the study area.

 

 

POSTER 14

Detection of Invasive Crayfish Populations by Environmental DNA in Fishponds from the Natural Park of Brenne

Mauvisseau Q, Coignet A, Delaunay C, Pinet F, Bouchon D and Souty-Grosset C

Environmental DNA (eDNA) is a powerful method for assessing the presence and the distribution of invasive aquatic species. We used this tool in order to detect and monitor several invasive crayfishes Procambarus clarkii, Orconectes limosus and Pacifastacus leniusculus,/i> present or likely to invade the fishponds of the Natural Regional Park of Brenne, one of the most important wetland in France and listed as an International RAMSAR wetland zone since 1991 We designed specific primers for each crayfish species, and set up an experimental aquarium approach to confirm the specificity of the primers and the sampling protocol. We analysed samples taken in the Natural Regional Park of Brenne in 2014 and 2015. The field experiment has proven the reliability of the eDNA detection method. Both experiments confirm that qPCR using SybrGreen protocol with the same primers give better reliable results that with TaqMan protocol. After optimization of the eDNA detection in water samples, it is concluded that sampling must be made during the main period of activity of crayfish, i.e. in summer. This method is a powerful tool for establishing the presence or absence of invasive species in the numerous ponds (more than 2000) in the National Regional Park of Brenne.

 

 

POSTER 15

Reproductive Cycle of the Marble Crayfish from an Established Population in Croatia

Cvitanić M, Hudina S and Maguire I

The marble crayfish (Procambarus fallax f. virginalis) is a relatively new non-indigenous crayfish species that has established populations in Europe. Until today, it is the only known decapod species with parthenogenetic reproduction. Established population of marble crayfish was recently discovered in a gravel pit in Northwest Croatia. Apart from parthenogenetic reproduction, the invasion success of this species stems from its early maturation, and higher fecundity compared to other crayfish species. Therefore, the aim of this study was to examine the year cycle of the marble crayfish in Croatia, and its potential (number of ovarian eggs) and realized fecundity (number of pleopodal eggs and juveniles). Our results show that all 140 examined individuals were reproductively active during the whole sampling period. Peaks of reproduction activity were recorded from September to November, when both individuals with pleopodal eggs or juveniles as well as individuals with ripe ovarian eggs were recorded. The smallest reproductively active female was 40.81 mm TL, while reproductive output increased with size (TL). The average number of ovarian eggs was 297. The average number of pleopodal eggs was 15% lower than the number of ovarian eggs, while number of juveniles attached to pleopods was reduced by 50% from ovarian egg count. This represents a lower brood loss compared to other crayfish species. Due to almost constant reproduction, early maturation and relatively high potential and realized fecundity in an established population in Croatia, the marble crayfish represents a major threat to the surrounding waterbodies and their native crayfish fauna.

 

 

POSTER 18

No Species Recognition Between Two Alien Crayfish Species?

Tricarico E, Coignet A and Souty-Grosset C

Crayfish rely on odours for finding a resource, detecting predators and recognizing species. Species that are not co-evolved could not chemically recognize each other. The red swamp crayfish Procambarus clarkii and the spinycheek crayfish Orconectes limosus, both native to North America where they occupy different areas, have coexisted in the “Parc Naturel Régional de la Brenne” (Centre Region, France) since 2007 but in different ponds. Behavioural observations were conducted in October 2014 and April 2015 to assess if P. clarkii and O. limosus are able to chemically recognize individuals of both sexes belonging to the same or other species. In experimental individual aquarium, records of behaviour of 20 Procambarus males and 20 Orconectes males were made of 3-min observation bouts for each of two sequential phases: (a) the “water” phase, following the introduction of 10 mL of well water, (b) the “smell” phase, following the introduction of 10 mL of well water conditioned by (1) P. clarkii male, (2) P. clarkii female, (3) O. limosus male and (4) O. limosus female odour. The time spent by each crayfish in locomotion and other activities (feeding, cleaning), and the time spent in one of three postures (raised, intermediate, or lowered) were recorded every 15 s. Crayfish seem able to recognize the conspecific of the same sex (males), and not the heterospecific, underlying the absence of species recognition in these two species that separately evolved.

 

 

POSTER 24

Effect of Pharmaceuticals on Crayfish

Shaliutina O and Bláha M

The release of human pharmaceuticals and personal care products into aquatic ecosystems continues to be a serious environmental problem. There is a staggering list of pharmaceuticals that have been detected in surface water, groundwater and tap water. These compounds typically modify the physiology or behavior, of the intended target system, without lethal effect. The wide range of entry points into freshwater systems reaffirms that animals living within those habitats face continuous exposure to pharmaceuticals, even if only at low doses. Crayfish are a crucial invertebrate in freshwater ecosystems. They are omnivorous, and occupy a key position in the trophic web as both predator and prey. With the importance of crayfish to their ecosystems, the introduction of pollutants such as pharmaceuticals, could have negative effects to crayfish populations. For example, pharmaceuticals can cause a mediating aggressive behavior. It has been demonstrated that crayfish injected with serotonin (antidepressant) fights lasted considerably longer. Also, the lasting impact of pharmaceuticals leads to immune system function decline, which greatly increases the mortality rate. Moreover, they can slow down synthesize and release of hormones from the X-organ sinus complex, and thus influence molting, gonad development, water balance, blood glucose, etc. However, despite all data the impact of pharmaceuticals on particular developmental stages, mortality, growth rate and postembryonic development of crayfish had not yet been fully explored. Therefore, we provide an overview about the known effects of pharmaceuticals on crayfish and demonstrate that these effects could be quite multifaceted.