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LIST OF MEETING ABSTRACTS

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Oral | Posters

Oral Presentations

 

 

Day: 3, Session: 2, Talk: 2

How do Invasive Species Cope with Different Environmental Conditions? A Proteomic Study of two Alien Crayfish: Procambarus clarkii and Procambarus fallax f. virginalis

Francisco J. Oficialdegui, Roessink I, Biron D, Boyero L, Clavero M, Peeters ETHM and Sánchez MI

Invasive species are often able to cope with variety environmental conditions enabling them to expand to new habitats. Once introduced and successfully established those invasive species, often threaten native species in their ecosystems. New environmental conditions may act as selective pressures that drive organismal adaptation and evolution. However, alongside genetic adaptation, invasive organisms also show a high degree of plasticity in response to environmental challenge. To elucidate whether epigenetic mechanisms allow an organism to respond to the environment, we study how individuals of two invasive crayfish species respond to environmental challenges by means of a cutting-edge proteomic approach. Our objective is to investigate which types of proteins and how many of each type are expressed when individuals are exposed to different environmental conditions. Our target species are two invasive crayfish, i.e. the red swamp crayfish, Procambarus clarkii; and the parthenogenetic marbled crayfish, Procambarus fallax f. virginalis. The use of a parthenogenetic species will allow us to assess whether changes in protein expression are uniquely due to plasticity (as individuals are genetically identical). We conduct an experiment in which we expose the two crayfish species to treatments consisting of different combinations of temperature and water velocity for several days. After exposure, individuals will be collected and immediately subjected to tissue extraction from the muscle, gills and hepatopancreas to examine the proteome responses. Here, we will present the details of the experimental setup and hypotheses tested as well as any available results.

 

Poster Presentations

 

 

POSTER 3

White-clawed Crayfish (Austropotamobius pallipes) Endangered Species Sampling Methods: Efficiency and Disruptions in the Balance

Olarte N, García-Arberas L and Alvaro A

The distribution of Austropotamobius pallipes in Biscay (Basque Country) is being increasingly restricted. After crayfish plague and the effects of many further threats, this specie is found in small headwater creeks away from the main streams. The species inhabits in small populations, generally isolated and fluctuating number of individuals. The main characteristic of these river stretches with a little flow is that they do not allow trap sampling methodology and so, other methods based on night viewing are used to get data to calculate population estimates. Two small rivers were sampled for 5 years. In this study, we have compared the values obtained by night viewing from river-bank (CPUEs) with manual searching on one pass (CPUEc). We have also correlated these methods with the estimate population size obtained by Removal method (ABUNDANCE). Both indirect estimates were highly correlated with ABUNDANCE, being stronger CPUEs-ABUNDANCE relationship (p=0.80; R2 =0.64) than CPUEc-ABUNDANCE one (p=0.67; R2 = 0.46). Night viewing is an acceptable strategy for population estimates without a direct interaction with the individuals. This strategy may optimize sampling, as it lets increasing the efficiency in effort and time and minimizing the perturbation generated with catching activity.

 

 

POSTER 8

Fine Structure of the Spermatozoon in Three Species of (Arthropoda: Crustacea: Decapoda) Cambarus robustus, Orconectes propinquus and Orconectes rusticus: A Comparative Biometrical Study

Yazicioglu B, Hamr P, Kozák P, Kouba A and Niksirat H

The ultrastructure of spermatozoa in three species of cambarid crayfish, including Cambarus robustus, Orconectes propinquus, and Orconectes rusticus were studied and compared with eight previously studied species from different crayfish families using morphological features and biometrical data. The ultrastructure of spermatozoa show a generally conserved pattern including an acrosome and nucleus in the anterior and posterior parts of the cell, respectively, radial arms that wrap around the nucleus, and the whole cell is enclosed by an extracellular capsule. The most outstanding morphological feature in spermatozoa of three studied cambarid crayfish is the crest-like protrusions in the anterior part of the acrosome that can be used as one of the features for distinguishing the members of this family. Results of biometrical data reveal that acrosome size in the representatives of Parastacidae are the smallest, while representatives of Astacidae show the biggest acrosome. The acrosome size in species belonging to Cambaridae occupy an intermediate position between the two other families of freshwater crayfish. In conclusion, a combination of morphological features and biometrical data of spermatozoa can provide an effective tool to distinguish different species of the freshwater crayfish.

 

 

POSTER 9

Metabolic Rate of Cambarellus montezumae (Crustacea: Cambaridae): Effects of Size and Seasonal Changes

Latournerié-Cervera JR, García-Padilla GM, and Estrada-Ortega AR

The measurement of metabolic rate is a key element of a species energy balance. The evaluation of respiratory metabolism in natural conditions, allows us to know the energy requirement of the animal for different processes including growth, activity, reproduction, among others. The objective of this study was to know the element (R) of the energy budget equation: C = P+R+F+U (IBP, 1968) in Cambarellus montezumae, during hot rainy season (May – August) and transitional months (September – October) in the area of study. Recently collected samples of crayfish (n=15) representing small, medium and large sizes of both sexes were employed in respirometry experiments, using a closed respirometer. The temperature and water quality were similar to the conditions of the sampling site. Two measurement periods were used: (noon and evening). Average routine metabolic rate was used for comparisons (QO₂: mg O₂/g. dry weight/h) and was converted to cal/g.dw/day using the Q_ox=3.31 cal/mgO₂ (Brafield & Solomon, 1972). Comparisons for size and month were made by ANOVA factorial (3 X 6) and Tukey test post hoc (p<0.05). Significant differences were obtained by month and size. QO₂ was similar between the months of the rainy season, with a temperature average range of (22.3 ± 1.6°C) and had a significant decrease (p<0.05) in October. Relationships of QO₂ – WW were calculated for all months using a potential model. We discuss results in relation to energy requirements of the species for growth and reproduction and its potential use for intensive management.

 

 

POSTER 10

The Impact of Streetlights on an Aquatic Invasive Species: Artificial Light at Night Alters Signal Crayfish Behaviour

Thomas JR, James J, Newman RC, Riley WD, Griffiths SW and Cable J

Artificial light at night (ALAN) can alter the behaviour, communication and orientation of animals, and could interact with other stressors to affect biodiversity. Invasive, non-native species are one of the largest threats to freshwater biodiversity; however, the impact of ALAN on such species is unknown. This study assessed the effects of ALAN at ecologically relevant levels on the behaviour of a globally widespread invasive species, the signal crayfish (Pacifastacus leniusculus). Crayfish were exposed to periods of daylight, control (<0.1 lx) and street-lit nights to test two hypotheses: (1) signal crayfish under natural conditions are nocturnal animals and (2) ALAN reduces the duration of crayfish activity and intraspecific interactions, whilst increasing their propensity to use shelter. Our results confirm that signal crayfish are largely nocturnal, showing peak activity and interaction levels during control nights, whilst taking refuge during daylight hours. However, when exposed to ecologically relevant simulated light pollution from a streetlight at night, activity and interactions with conspecifics were significantly reduced, whilst time spent sheltering increased. Global anthropogenic changes such as ALAN may alter the life history traits and behaviour of invasive species and ultimately influence their impact on invaded ecosystems. The results of the present study suggest that ALAN could reduce the success of signal crayfish in urban areas, by drastically reducing their nocturnal activity. This study is the first to show an impact of ALAN on the behaviour of an invasive, non-native species, and provides information for the management of invasive crayfish in areas where ALAN is prevalent.

 

 

POSTER 13

Some Aspects of the Dynamic Population of a Mexican Crayfish Species, Cambarellus montezumae (Saussure) from Xochimilco

Latournerié-Cervera JR, García-Padilla GM, Estrada-Ortega AR and Arana-Magallón F

At Xochimilco's Lake, still remains a wild population of Cambarellus montezumae crayfish. This population has been declining in the last decades, because of fragmentation and loss of habitat, overexploitation, pollution, competition and predation by alien species. In this study, we analyze dynamic population of this species through indicators of abundance, meristic indexes (wet weight, WW; total length, TL; and cephalothorax length, CTL); frequency of TL distributions, cohort´s growth rate, sex ratio and allometric relationships in crayfishes of both sexes. A total of 1345 C. montezumae crayfish, were collected during a twelve-month sampling, at Xochimilco channels in 2011 annual cycle. W.W. varied between (.01 – 1.53) g, and TL (6.47 – 41.21) mm, significant differences (p<0.05) were found by sex and month. Abundance was higher during hot rainy season (April – August). Significant differences were detected in total sex ratio population (Female:Male) being almost (2:1). Allometric relationships: WW- TL and WW - CTL - were better adjusted by a potential model, and CTL – TL by a linear model. Two cohorts were identified through the annual cycle (the one at April and second in August), and growth rate was calculated for both of them using TL frequency distributions. We discuss dynamic population of this species in relation to habitat changes and anthropogenic disturbances, which have led almost to its collapse and disappearance.

 

 

POSTER 18

No Species Recognition Between Two Alien Crayfish Species?

Tricarico E, Coignet A and Souty-Grosset C

Crayfish rely on odours for finding a resource, detecting predators and recognizing species. Species that are not co-evolved could not chemically recognize each other. The red swamp crayfish Procambarus clarkii and the spinycheek crayfish Orconectes limosus, both native to North America where they occupy different areas, have coexisted in the “Parc Naturel Régional de la Brenne” (Centre Region, France) since 2007 but in different ponds. Behavioural observations were conducted in October 2014 and April 2015 to assess if P. clarkii and O. limosus are able to chemically recognize individuals of both sexes belonging to the same or other species. In experimental individual aquarium, records of behaviour of 20 Procambarus males and 20 Orconectes males were made of 3-min observation bouts for each of two sequential phases: (a) the “water” phase, following the introduction of 10 mL of well water, (b) the “smell” phase, following the introduction of 10 mL of well water conditioned by (1) P. clarkii male, (2) P. clarkii female, (3) O. limosus male and (4) O. limosus female odour. The time spent by each crayfish in locomotion and other activities (feeding, cleaning), and the time spent in one of three postures (raised, intermediate, or lowered) were recorded every 15 s. Crayfish seem able to recognize the conspecific of the same sex (males), and not the heterospecific, underlying the absence of species recognition in these two species that separately evolved.

 

 

POSTER 20

Novel Microsporidian Infection in the Japanese Endemic Crayfish Cambaroides japonicus

Pretto T, Tanaka, Diéguez-Uribeondo J and Kawai T

The endemic crayfish, Cambaroides japonicus (de Haan, 1841) is the only native crayfish species in Japan. The rapid decreasing of its native range, which comprises Hokkaido and the Northern Honshu, led the Environmental Agency in Japan to consider C. japonicus as an endangered species. During a monitoring survey on the distribution of C. japonicus,/i> in Hokkaido in 2011, a specimen with atypical whitish appearance of the abdominal musculature were observed in Lake Toyoni and porcelain disease (Thelohania sp.) has been suspected. A focused survey conducted in Lake Toyoni in 2013 revealed the presence of macroscopically affected crayfish with a prevalence of 6.3%. Ethanol fixed C. japonicus were analyzed and muscle samples for molecular analysis were taken from 8 affected specimens. The small subunit ribosomal DNA (SSU rDNA), amplified with generic microsporidian primers (Weiss and Vossbrinck, 1998), was sequenced and compared with other marine, freshwater and terrestrial Microsporidia. Histological analysis of the abdomen revealed high densities of ovoid single spores, not contained inside a sporophorous vescicle, in striated muscle cells of the pleonal extensor and flexor muscles. No spores were recorded in the intestinal musculature nor in the ventral ganglia. The affected muscle fibers were frequently surrounded by haemocytic infiltration and sporadic melanization. The phylogenetic analysis of the SSU rDNA placed this undescribed microsporidium between members of the class Marinosporidia (Vossbrinck and Debrunner-Vossbrinck, 2005), distant from the microsporidia of other European and Australian crayfish. Further studies are needed to describe the developmental stages and the ultrastructural features of this microsporidium.

 

 

POSTER 21

Genetic Diversity and Differentiation of Wild Populations and Captive Stocks of the Noble Crayfish (Astacus astacus L.) in Estonia

Gross R and Kaldre K

There are about 20 crayfish farms in Estonia which produce and sell noble crayfish for restocking and enhancement of natural waterbodies and/or for human consumption. So far, the origin of captive stocks and their genetic characteristics have not been studied and likewise, there is lack of information about genetic diversity and population structure in a wild. The aim of this study was to evaluate genetic diversity and population genetic structure of captive stocks and wild populations of the noble crayfish in Estonia. A total of 1923 individuals from six crayfish farms and 38 natural lakes and rivers in Estonia were analyzed using 19 polymorphic tetranucleotide repeat microsatellite markers. For comparison, two populations from Czech Republic were included. The results showed that Estonian noble crayfish populations were on an average less variable than the Czech populations and formed two clear genetic clusters according to their geographic origin (Island of Saaremaa and mainland Estonia). The captive stocks were genetically similar to the wild populations of the same region and displayed no significant loss of genetic variability. However, two captive stocks possessed increased levels of inbreeding. The results of the study will be used for improving conservation and management plans of the noble crayfish in Estonia.

 

 

POSTER 22

Positive Selection of Beautiful Invasives: Long-term Persistence and Bio-invasion Risk of Ornamental Crayfish

Chucholl C and Wendler F

Following a distinct peak interest to keep freshwater crayfish in home aquaria in the mid-2000s, the aquarium trade has become a novel introduction pathway for alien crayfish species in Central Europe. Here, we provide an update on the German ornamental crayfish trade approximately one decade after the `crayfish hype´ to explore the long-term implications in terms of bio-invasion risk. Specifically, species’ availability and potential invasiveness, as well as the determinants of availability were assessed. In July 2015, a total of 31 online shops offered 28 crayfish species, which represents a decline of 24% in species diversity compared to the late 2000s. In addition, the estimated rate of import of new species has considerably flattened and approaches pre-hype values (< 1 species · y^-1). However, the risk associated with the offered species, as assessed by a risk screening tool (FI-ISK), has not decreased compared to the late 2000s. Long-term availability in the trade (covering one decade) was primarily determined by bright coloration, the ability to reproduce under warm aquarium conditions, and a preference for lentic habitats. Species featuring such traits are likely to persist in the aquarium trade and include four high-risk species, most notably invasive and crayfish plague-carrying red swamp crayfish (Procambarus clarkii) and Marmorkrebs (Procambarus fallax f. virginalis). Persistent propagule pressure from aquaria has substantially contributed to the establishment of both species in Central Europe, stressing the need for effective pathway management.

 

 

POSTER 24

Effect of Pharmaceuticals on Crayfish

Shaliutina O and Bláha M

The release of human pharmaceuticals and personal care products into aquatic ecosystems continues to be a serious environmental problem. There is a staggering list of pharmaceuticals that have been detected in surface water, groundwater and tap water. These compounds typically modify the physiology or behavior, of the intended target system, without lethal effect. The wide range of entry points into freshwater systems reaffirms that animals living within those habitats face continuous exposure to pharmaceuticals, even if only at low doses. Crayfish are a crucial invertebrate in freshwater ecosystems. They are omnivorous, and occupy a key position in the trophic web as both predator and prey. With the importance of crayfish to their ecosystems, the introduction of pollutants such as pharmaceuticals, could have negative effects to crayfish populations. For example, pharmaceuticals can cause a mediating aggressive behavior. It has been demonstrated that crayfish injected with serotonin (antidepressant) fights lasted considerably longer. Also, the lasting impact of pharmaceuticals leads to immune system function decline, which greatly increases the mortality rate. Moreover, they can slow down synthesize and release of hormones from the X-organ sinus complex, and thus influence molting, gonad development, water balance, blood glucose, etc. However, despite all data the impact of pharmaceuticals on particular developmental stages, mortality, growth rate and postembryonic development of crayfish had not yet been fully explored. Therefore, we provide an overview about the known effects of pharmaceuticals on crayfish and demonstrate that these effects could be quite multifaceted.

 

 

POSTER 28

Management of the Native White-clawed Crayfish, Austropotamobius pallipes, in the Province of Girona

Montserrat J, Macias M and Torres F

The native white-clawed crayfish (Austropotamobius pallipes) was a species commonly found in most watercourses in the basins of the rivers Fluvià, Ter and Muga until the end of the 1970s, when the crayfish plague Aphanomyces astaci arrived, a water mould that led to the disappearance of almost all the populations in these river basins. The white-clawed crayfish populations are currently found in second-order or larger seasonal rivers and fast-flowing streams where, due to some kind of natural barrier (drought in the lower reaches of the watercourse, cliffs, etc.), the fungus was unable to grow. Moreover, during the 1990s, the red swamp crayfish (Procambarus clarkii) population expanded rapidly in the basin and the situation became far worse, since the North American species is both a carrier of the crayfish plague and resistant to this fungal disease. Later, the signal crayfish (Pacifastacus leniusculus) arrived in the Ter River and the spinycheek crayfish (Orconectes limosus) arrived in the Muga River Basin, both species being carriers of the pathogen Aphanomyces astaci. This situation means relict populations of the white-clawed crayfish cannot expand and are forced to survive in the most inaccessible seasonal rivers and fast-flowing streams, facing the danger of a new outbreak of crayfish plague.