Abstracts

IAA21 Book of Abstracts (PDF)

Meeting Program/Schedule (PDF)

 VIEW MEETING PROGRAM ONLINE

LIST OF MEETING ABSTRACTS

NOTE: You may quickly navigate to a name you are looking for by clicking a letter below (first letter in their lastname).

Oral | Posters

Oral Presentations

There are no oral presentations by the currently selected author.

Poster Presentations

POSTER 14

Detection of Invasive Crayfish Populations by Environmental DNA in Fishponds from the Natural Park of Brenne

Mauvisseau Q, Coignet A, Delaunay C, Pinet F, Bouchon D and Souty-Grosset C

Environmental DNA (eDNA) is a powerful method for assessing the presence and the distribution of invasive aquatic species. We used this tool in order to detect and monitor several invasive crayfishes Procambarus clarkii, Orconectes limosus and Pacifastacus leniusculus,/i> present or likely to invade the fishponds of the Natural Regional Park of Brenne, one of the most important wetland in France and listed as an International RAMSAR wetland zone since 1991 We designed specific primers for each crayfish species, and set up an experimental aquarium approach to confirm the specificity of the primers and the sampling protocol. We analysed samples taken in the Natural Regional Park of Brenne in 2014 and 2015. The field experiment has proven the reliability of the eDNA detection method. Both experiments confirm that qPCR using SybrGreen protocol with the same primers give better reliable results that with TaqMan protocol. After optimization of the eDNA detection in water samples, it is concluded that sampling must be made during the main period of activity of crayfish, i.e. in summer. This method is a powerful tool for establishing the presence or absence of invasive species in the numerous ponds (more than 2000) in the National Regional Park of Brenne.