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POSTER 6

Epifauna on Freshwater Crayfish (Crustacea: Decapoda) in Croatia

Dražina T, Maguire I, Korša A, Špoljar M and Klobučar G

The crustacean exoskeleton is well known substratum for associate species - epibionts. During 2014 and 2015, we have studied epifauna on six different freshwater crayfish species collected from continental and mediterranean regions of Croatia (Europe). Four of them are autochthonous (Astacus astacus, Astacus leptodactylus, Austropotamobius pallipes, Austropotamobius torrentium) and two allochthonous invasive species (Pacifastacus leniusculus and Procambarus fallax f. virginalis). The epibiont samples were collected from the exoskeleton surface and from the crayfish gill cavity. The aims of this research were: (i) to analyse epifaunal assemblage on different freshwater crayfish and (ii) to compare epifauna among different crayfish populations, especially between autochthonous and allochthonous species. A total of 44 different epifaunal taxa were recorded. The most abundant group was Ciliophora, with Vorticella campanula and Epistylis sp. as the most frequent taxa. Rotifera was the most diverse group and 16 different taxa were identified. Four rotifer species (Lepadella astacicola, L. branchiola, L. parasitica and Dicranophorus hauerianus) were determinated as specific inhabitants of certain crayfish species and these rotifers were found exclusively on autochthonous species. Branchiobdellidans were constant crayfish epibionts, and for the first time the North American species Xirogiton victoriensis has been recorded in Croatia. Representatives from Catenulida, Gastrotricha, Nematoda, Bivalvia, Hirudinea, Tardigrada, Crustacea, Hydrachnidia and Chironomidae were also recorded as epibionts on freshwater crayfish. Results of this study suggested the separation of epifaunal assemblage between autochthonous and allochthonous crayfish, as well as the separation of epifauna among autochthonous species. Our results indicate highly diverse, complex and specific epifaunal assemblage on different crayfish populations. Thus, multiple relationships between crayfish host and epibionts will be the topic of further studies.

 

 

POSTER 8

Fine Structure of the Spermatozoon in Three Species of (Arthropoda: Crustacea: Decapoda) Cambarus robustus, Orconectes propinquus and Orconectes rusticus: A Comparative Biometrical Study

Yazicioglu B, Hamr P, Kozák P, Kouba A and Niksirat H

The ultrastructure of spermatozoa in three species of cambarid crayfish, including Cambarus robustus, Orconectes propinquus, and Orconectes rusticus were studied and compared with eight previously studied species from different crayfish families using morphological features and biometrical data. The ultrastructure of spermatozoa show a generally conserved pattern including an acrosome and nucleus in the anterior and posterior parts of the cell, respectively, radial arms that wrap around the nucleus, and the whole cell is enclosed by an extracellular capsule. The most outstanding morphological feature in spermatozoa of three studied cambarid crayfish is the crest-like protrusions in the anterior part of the acrosome that can be used as one of the features for distinguishing the members of this family. Results of biometrical data reveal that acrosome size in the representatives of Parastacidae are the smallest, while representatives of Astacidae show the biggest acrosome. The acrosome size in species belonging to Cambaridae occupy an intermediate position between the two other families of freshwater crayfish. In conclusion, a combination of morphological features and biometrical data of spermatozoa can provide an effective tool to distinguish different species of the freshwater crayfish.


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