none none 00222011 Journal of Invertebrate Pathology Journal of Invertebrate Pathology Molecular detection of Aphanomyces astaci – An improved species specific qPCR assay David A. Strand Tomas Jinnerot Anna Aspán Satu Viljamaa-Dirks Sirpa Heinikainen Elin Rolén Trude Vrålstad 201 108008 108008 2023 full_text 10.1016/j.jip.2023.108008 FRESHWATER CRAYFISH: Prevalence of the crayfish plague, <i>Aphanomyces astaci</i> Schikora, in alien crayfish species in Japan
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Freshwater Crayfish 25(1): 31-37 (2020)

PEER REVIEWED    RESEARCH ARTICLE

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Prevalence of the crayfish plague, Aphanomyces astaci Schikora, in alien crayfish species in Japan

Kamimura K and Kawai T  e-mail link

Published Online: 4/15/2020

Abstract

Crayfish plague is a severe disease of crayfish that is caused by the oomycete Aphanomyces astaci. Two crayfish hosts of this parasite, Procambarus clarkii and Pacifastacus leniusculus, were imported from North America into Japan and were found to be infected with this parasite. Since the endemic Japanese crayfish, Cambaroides japonicus, has a low resistance to the crayfish plague, infection with this parasite will likely lead to crayfish death. Specimens of both invasive crayfish species were collected across Japan and their infection status was examined using a PCR technique. Aphanomyces astaci was detected in all localities and the average infection prevalence was 67%. Additionally, when the signs of melanization were compared with the results from PCR analyses, it suggested that the DNA detection procedure is more reliable than observation of tissue melanization. Moreover, the relationship between prevalence and water temperature in the field was analyzed, indicated that water temperature influenced the prevalence of A. astaci infection.

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How to Cite

Kamimura K and Kawai T. (2020). Prevalence of the crayfish plague, Aphanomyces astaci Schikora, in alien crayfish species in Japan. Freshwater Crayfish 25(1):31-37. doi: 10.5869/fc.2020.v25-1.031

 

 

Author Information

Tadashi  Kawai,* Central Fisheries Research Institution, Hokkaido Research Organization, 238 Hamanaka, Yoichi, Hokkaido, Japan046-8555. E-mail: tadashikawai8@gmail.com

Kensuke   Kamimura, Department of Natural Environmental Studies , The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, Japan277-8563. E-mail: kawai-tadashi@hro.or.jp

Corresponding Author indicated by an *.

 

Publication History

   Manuscript Submitted: 1/11/2019

   Manuscript Accepted: 1/18/2020

   Published Online: 4/15/2020

   Published in Print: 4/30/2020

 

 

Funding Information

No specific funding statement is available for this article.

 

 



 

 

 

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