none none 20751508 23129581 Ribogospodarsʹka nauka Ukraïni. Ribogospod. nauka Ukr. Biology and cultivation of freshwater crayfish (Astacoidea) in Ukraine and worldwide. Thematic list of scientific publications (2019–2024) R. Sydorak 2(68) 164 2024 full_text 10.61976/fsu2024.02.164 2296-889X Frontiers in Molecular Biosciences Front. Mol. Biosci. Detecting Sex-Related Changes to the Metabolome of a Critically Endangered Freshwater Crayfish During the Mating Season Emily D. Lette Quinton F. Burnham Nathan Lawler Pierre Horwitz Mary C. Boyce David I. Broadhurst Rodney Duffy Annette Koenders 8 650839 2021 full_text 10.3389/fmolb.2021.650839 2296-7745 Frontiers in Marine Science Front. Mar. Sci. Transcriptome and metabolome analysis of intersex gonads of redclaw crayfish (Cherax quadricarinatus) Ruiqi He Chuntai Nong Yibin Chen Sedong Li Huapu Chen 11 1506646 2024 full_text 10.3389/fmars.2024.1506646 FRESHWATER CRAYFISH: Metabolomic profiling of crayfish haemolymph distinguishes sister species and sex: implications for conservation, aquaculture and physiological studies
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Freshwater Crayfish 25(1): 89-101 (2020)

PEER REVIEWED    RESEARCH ARTICLE

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Metabolomic profiling of crayfish haemolymph distinguishes sister species and sex: implications for conservation, aquaculture and physiological studies

Lette ED, Lawler NG, Burnham QF, Boyce MC, Duffy R, Koenders A and Broadhurst DI   e-mail link

Published Online: 4/15/2020

Abstract

Hairy marron, Cherax tenuimanus (Smith) are critically endangered freshwater crayfish found only in a single river in southwest Australia. Conservation efforts have included a captive breeding program, which has been largely unsuccessful, despite the closely related smooth marron, Cherax cainii Austin, being successfully bred for aquaculture. Using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomic approach we created a profile of the metabolites in the haemolymph for males and females of the two species of marron. A non-lethal method was used to collect haemolymph and 84 reproducible annotated metabolites were identified. Variation in the levels of some metabolites were detected between species and between sexes within species. Multivariate analyses clearly differentiated the congeneric species and univariate analyses identified differences between species, sex, and for some metabolite interactions, between species and sex. This study created a baseline metabolome dataset for the two species and began to investigate the biological significance of metabolites that varied between species. We have shown metabolomics could be used for targeted studies to potentially assist reproductive success. This approach will be beneficial for conservation and aquaculture practices with potential applications for other aquatic taxa worldwide.

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Lette ED, Lawler NG, Burnham QF, Boyce MC, Duffy R, Koenders A and Broadhurst DI . (2020). Metabolomic profiling of crayfish haemolymph distinguishes sister species and sex: implications for conservation, aquaculture and physiological studies. Freshwater Crayfish 25(1):89-101. doi: 10.5869/fc.2020.v25-1.089

 

 

Author Information

Emily D. Lette,* School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup , Western Australia, Australia6027. E-mail: e.lette@ecu.edu.au

Nathan G. Lawler, School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia, Australia6027. E-mail: n.lawler@ecu.edu.au

Quinton F. Burnham, School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia, Australia6027. E-mail: q.burnham@ecu.edu.au

Mary C. Boyce, School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia, Australia6027. E-mail: m.boyce@ecu.edu.au

Rodney  Duffy, Fisheries, State Government of Western Australia, Department of Primary Industries and Regional Development, 39 Northside Dr, Hillarys, Western Australia, Australia6025. E-mail: rodney.duffy@dpird.wa.gov.au

Annette  Koenders, School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia, Australia6027. E-mail: a.koenders@ecu.edu.au

David I. Broadhurst, School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, Western Australia, Australia6027. E-mail: d.broadhurst@ecu.edu.au

Corresponding Author indicated by an *.

 

Publication History

   Manuscript Submitted: 5/3/2019

   Manuscript Accepted: 1/7/2020

   Published Online: 4/15/2020

   Published in Print: 4/30/2020

 

 

Funding Information

No specific funding statement is available for this article.

 

 



 

 

 

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