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Freshwater Crayfish 12(1): 313-318 (1999)


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RAPD evidence for the origin of an outbreak of crayfish plague in Spain

Diéguez-Uribeondo J and Söderhäll K  e-mail link

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A strain of the crayfish plague fungus Aphanomyces astaci, Pa, responsible for a recent outbreak of crayfish plague in Austropotamobius pallipes in the Caragua River Basin, Burgos, Spain, was characterised by applying DNA finger printing technique, RAPD-PCR. The strain exhibits an identical DNA band pattern to a strain earlier isolated from the signal crayfish Pacifastacus leniusculus. The results obtained by RAPD-PCR were confirmed by data obtained from a physiological characterisation of the spores of the strain Pa. This study clearly shows that introductions of signal crayfish into Spain have resulted in the transmission of the crayfish plague fungus from signal crayfish to the native crayfish, A. pallipes.

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Diéguez-Uribeondo J and Söderhäll K. (1999). RAPD evidence for the origin of an outbreak of crayfish plague in Spain. Freshwater Crayfish 12(1):313-318. doi:



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